Subcellular Localization
Visualize protein localization in plant cells using fluorescent protein tagging. Supports confocal microscopy for precise cellular compartment identification.
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Yeast Two-Hybrid (Y2H)
Screen protein-protein interactions using yeast two-hybrid libraries. Identify new interaction partners for your target proteins.
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Co-Immunoprecipitation (Co-IP)
Detect protein complexes in plant tissues or cell extracts, allowing confirmation of in vivo interactions.
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IP-MS
Identify interacting proteins using immunoprecipitation coupled with mass spectrometry for comprehensive interactome analysis.
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BiFC (Bimolecular Fluorescence Complementation)
Visualize protein-protein interactions in living plant cells using split fluorescent proteins for spatial interaction analysis.
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EMSA
Electrophoretic mobility shift assay to detect protein-DNA/RNA interactions. Analyze transcription factor binding specificity and affinity.
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Yeast One-Hybrid (Y1H)
Screen transcription factor binding to DNA elements using yeast one-hybrid libraries. Ideal for promoter analysis and regulatory network studies.
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Dual-Luciferase Assay
Measure promoter activity and transcriptional regulation using two luciferases. Useful for validating regulatory elements in plant systems.
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LCA (Luciferase Complementation Assay)
Detect protein-protein or protein-DNA interactions via split luciferase complementation. Monitor real-time interactions in living cells.
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