1. Service Overview
Co-Immunoprecipitation (Co-IP) is a classical and powerful technique used to study protein–protein interactions in plant and animal systems. It allows researchers to validate physical interactions between target proteins, helping to elucidate signaling pathways, protein complexes, and regulatory networks.
YBioHub provides professional Co-IP services with both plant and animal cell systems, ensuring high-quality, reliable, and reproducible results.
2. Principle
Co-IP is based on antibody-mediated protein capture:
- Use a specific antibody to bind the target protein (bait).
- The antibody-protein complex is immobilized on beads (e.g., Protein A/G beads).
- Associated proteins (prey) that interact with the bait are co-precipitated.
- Eluted proteins are analyzed by Western blot or mass spectrometry to identify interactions.
3. Experimental Workflow (Simplified)
- Antibody validation & sample preparation
- Protein extraction and immunoprecipitation
- Washing and elution of complexes
- Detection by Western blot or mass spectrometry
- Data analysis
4. Applications
- Validation of protein–protein interactions
- Study of signal transduction pathways
- Investigation of protein complex formation
- Support for functional genomics and molecular biology studies
5. Service Advantages (YBioHub)
- 📌 Experienced team with multiple Co-IP systems
- 📌 Support for various antibodies and tags (FLAG, HA, Myc, GFP, etc.)
- 📌 High-quality detection via Western blot or mass spectrometry
- 📌 Fast turnaround, clear and reliable results
6. Deliverables
- Western blot images of precipitated proteins
- Interaction analysis report
- Raw data files (upon request)
7. FAQ
Q1: Which systems are commonly used for Co-IP?
A: Plant and animal cells, including tobacco, Arabidopsis, mammalian cultured cells, and yeast.
Q2: How long does the experiment usually take?
A: Typically about 1 month, depending on the system and project design.
Q3: Can the experiment be done without antibodies?
A: Yes. We provide commonly used antibodies and tags.
Q4: I only have the gene sequence, not the vector. Can I still order this service?
A: Yes. We provide a one-stop service from gene cloning to vector construction.
Q5: What will I receive as the final results?
A: You will receive Western blot images, an interaction analysis report, and raw data if requested.