1. Service Overview
Yeast One-Hybrid (Y1H) is a powerful technique to study transcription factor (TF) binding to specific DNA sequences. It is widely used to map promoter regions and regulatory elements in gene expression studies.
YBioHub provides professional Y1H services, from single-DNA fragment testing to large-scale screening, helping researchers quickly identify TF-DNA interactions and regulatory networks.
2. Principle
The target DNA fragment (promoter or regulatory element) is cloned into a reporter vector containing a detectable reporter gene (e.g., HIS3, LacZ, or GFP). The vector is transformed into yeast along with a vector expressing the transcription factor of interest. If the TF specifically binds the DNA fragment, the reporter gene is activated. Activation is detected by growth selection on selective media or enzymatic activity.
3. Experimental Workflow (Simplified)
- DNA fragment cloning: Clone the target DNA sequence into a reporter vector.
- Yeast transformation: Transform yeast with the reporter vector and the TF expression vector.
- Binding detection: Detect TF-DNA binding via reporter gene activation (growth selection or enzymatic assay).
- Quantitative analysis: Assess binding strength based on reporter gene expression to compare TF or DNA fragment binding capabilities.
4. Applications
- Identification of transcription factor-DNA interactions
- Promoter and regulatory element mapping
- Gene regulatory network analysis
- Screening candidate transcription factors for specific DNA sequences
5. Service Advantages (YBioHub)
- 📌 Professional team: experienced molecular biology and yeast experiment specialists
- 📌 Versatile systems: suitable for single-DNA fragment testing and DNA library screening
- 📌 High throughput & reliability: fast and accurate interaction results
- 📌 Complete data: deliver detailed experimental reports, images, and raw data
6. Deliverables
- Yeast growth or reporter activity images
- Detailed experimental analysis report
- Raw data files (upon request)
7. FAQ
Q1: I only have the DNA sequence, not the vector. Can I still order this service?
A: Yes. We provide a one-stop service from gene cloning to vector construction.
Q2: How long does the experiment usually take?
A: Typically about two months, depending on the experimental design and sample quantity.
Q3: Can multiple DNA fragments be screened simultaneously?
A: Yes. We offer DNA library screening to discover TF-DNA interactions efficiently.
Q4: What will I receive as the final results?
A: You will receive an experimental report, images of reporter activity, and raw data (if requested).
Q5: Do I need to provide a DNA library?
A: No. We have multiple cDNA libraries available for free use.