1. Service Overview
EMSA is a widely used technique to study DNA-protein interactions by detecting mobility shifts of DNA when bound by proteins. This method helps identify transcription factor binding and regulatory protein interactions with DNA.
YBioHub provides professional EMSA services, ensuring high-quality detection and reliable results with comprehensive reports.
2. Principle
The target DNA fragment is labeled and incubated with the protein of interest. If the protein binds the DNA, the DNA-protein complex shows reduced mobility during gel electrophoresis compared to free DNA. The shift is detected and analyzed to confirm binding specificity and strength.
3. Experimental Workflow (Simplified)
- DNA preparation: Label the DNA fragment of interest.
- Protein preparation: Express and purify the target protein or use cell extracts.
- Binding reaction: Incubate DNA with protein to form complexes.
- Electrophoresis: Separate DNA-protein complexes using native gel.
- Detection & analysis: Visualize shifts and quantify binding interactions.
4. Applications
- Identification of transcription factor-DNA binding
- Analysis of DNA-protein interaction specificity
- Validation of regulatory elements
- Functional characterization of protein-DNA complexes
5. Service Advantages (YBioHub)
- 📌 Experienced team: professional in molecular biology and DNA-protein interaction assays
- 📌 Flexible labeling: supports fluorescent or radioactive DNA labeling
- 📌 Reliable results: sensitive detection of DNA-protein complexes
- 📌 Complete deliverables: experimental report, gel images, and raw data
6. Deliverables
- EMSA gel images showing DNA-protein complexes
- Detailed experimental analysis report
- Raw data files (upon request)
7. FAQ
Q1: I only have the DNA sequence, not the vector. Can I still order this service?
A: Yes. We provide a one-stop service from gene cloning to vector construction.
Q2: How long does the experiment usually take?
A: Typically about 1 month, depending on sample type and design.
Q3: Can EMSA be performed without antibodies?
A: Yes. EMSA does not require antibodies; labeled DNA and proteins are sufficient.
Q4: What will I receive as the final results?
A: You will receive gel images, an experimental report, and raw data if requested.