1. Service Overview
IP-MS combines immunoprecipitation with mass spectrometry to study protein-protein interactions in complex biological samples. This approach is widely used to identify interacting partners and dissect protein complexes.
YBioHub provides professional IP-MS services, helping researchers discover protein interaction networks quickly and accurately, with comprehensive experimental reports.
2. Principle
The target protein is immunoprecipitated using a specific antibody or affinity tag. The captured protein complex is then analyzed by mass spectrometry to identify interacting proteins. This allows mapping of protein interaction networks and functional complexes.
3. Experimental Workflow (Simplified)
- Sample preparation: Cell or tissue lysis and protein extraction.
- Immunoprecipitation: Capture target protein using specific antibody or tag.
- Mass spectrometry analysis: Identify interacting proteins in the immunoprecipitated complex.
- Data analysis: Quantitative and qualitative analysis of protein interactions.
4. Applications
- Identification of protein-protein interactions
- Mapping of protein complexes
- Discovery of novel interacting partners
- Validation of functional protein networks
5. Service Advantages (YBioHub)
- 📌 Experienced team: professional in molecular biology, immunoprecipitation, and mass spectrometry
- 📌 Flexible system: supports antibodies, tags, or cDNA libraries
- 📌 Accurate & sensitive: high-quality mass spectrometry detection
- 📌 Complete deliverables: experimental report, MS data, and images
6. Deliverables
- Mass spectrometry data files
- Experimental analysis report
- Raw immunoprecipitation images (upon request)
7. FAQ
Q1: I only have the gene sequence, not the vector. Can I still order this service?
A: Yes. We provide a one-stop service from gene cloning to vector construction.
Q2: How long does the experiment usually take?
A: Typically about 1 month, depending on sample type and design.
Q3: Can this be done without antibodies?
A: Yes. We provide commonly used antibodies and tags.
Q4: What will I receive as the final results?
A: You will receive an experimental report, mass spectrometry data, and raw images (if requested).