1. Service Overview
The Luciferase Complementation Assay (LCA) is a powerful method for studying protein-protein interactions in living cells. By splitting luciferase into two fragments fused to proteins of interest, interaction reconstitutes luciferase activity, producing measurable bioluminescence.
YBioHub provides professional LCA services in plant and mammalian cell systems, delivering sensitive, quantitative, and real-time interaction detection.
2. Principle
The luciferase enzyme is split into two non-functional fragments. Each fragment is fused to a protein of interest. When the two proteins interact, the luciferase fragments reassemble and restore enzymatic activity, producing luminescence proportional to interaction strength.
3. Experimental Workflow (Simplified)
- Vector construction: Fuse luciferase fragments to proteins of interest.
- Cell transfection: Introduce fusion constructs into appropriate cell lines.
- Interaction detection: Measure luminescence with a luminometer, reflecting protein-protein interactions.
- Data analysis: Quantify luminescence to determine interaction strength and dynamics.
4. Applications
- Investigation of protein-protein interactions in vivo
- Functional analysis of signaling pathways
- Validation of candidate interacting proteins
- Screening compounds affecting protein interactions
5. Service Advantages (YBioHub)
- 📌 Professional team: experienced in molecular biology and live-cell interaction assays
- 📌 Sensitive and quantitative: detect weak and transient interactions
- 📌 Real-time detection: monitor dynamic interactions in living cells
- 📌 Comprehensive deliverables: detailed reports, luminescence images, and raw data
6. Deliverables
- Bioluminescence images and charts
- Comprehensive analysis report
- Raw luminescence data files (upon request)
7. FAQ
Q1: I only have the protein sequences, not the vectors. Can I still order this service?
A: Yes. We provide a one-stop service from vector construction to transfection and analysis.
Q2: How long does the experiment usually take?
A: Typically about one month, depending on cell system and experimental design.
Q3: Can multiple protein interactions be tested simultaneously?
A: Yes. Multiple protein pairs can be tested in parallel for comparative interaction analysis.
Q4: What will I receive as the final results?
A: You will receive experimental reports, luminescence charts, and raw data files (if requested).
Q5: Do I need to provide cell lines?
A: No. We offer commonly used plant and mammalian cell lines for your assays.