1. Service Overview
The Dual-Luciferase Reporter Assay is a widely used method for analyzing promoter activity, gene expression regulation, and transcription factor function. By co-transfecting a target promoter-driven luciferase reporter and a control luciferase, the assay provides highly sensitive and quantitative results.
YBioHub provides professional dual-luciferase assay services in plant and mammalian cell systems, offering reliable data and comprehensive analysis.
2. Principle
The assay uses two luciferase reporters: Firefly luciferase as the experimental reporter under the promoter of interest, and Renilla luciferase as an internal control for normalization. Luminescence signals are measured sequentially, allowing accurate quantification of promoter activity and transcriptional regulation.
3. Experimental Workflow (Simplified)
- Vector construction: Clone the promoter of interest upstream of firefly luciferase reporter.
- Cell transfection: Co-transfect cells with firefly reporter, Renilla control, and TF expression vector (if applicable).
- Luminescence detection: Measure firefly and Renilla luciferase activity using a dual-luciferase reader.
- Data analysis: Normalize firefly activity to Renilla activity to calculate relative promoter activity.
4. Applications
- Analysis of promoter activity and regulatory elements
- Transcription factor functional studies
- Signal pathway investigation
- Screening small molecules affecting gene expression
5. Service Advantages (YBioHub)
- 📌 Professional team: experienced in molecular biology and cell-based assays
- 📌 High sensitivity: dual-reporter normalization ensures reliable quantification
- 📌 Versatile: suitable for plant and mammalian cell systems
- 📌 Complete deliverables: detailed experimental reports, raw data, and images
6. Deliverables
- Luminescence measurement images and charts
- Comprehensive analysis report
- Raw luminescence data files (upon request)
7. FAQ
Q1: I only have the promoter sequence, not the vector. Can I still order this service?
A: Yes. We provide a one-stop service from vector construction to transfection and analysis.
Q2: How long does the experiment usually take?
A: Typically about one month, depending on cell system and experimental design.
Q3: Can different transcription factors be tested simultaneously?
A: Yes. Multiple TFs can be tested in parallel for comparative promoter activity analysis.
Q4: What will I receive as the final results?
A: You will receive experimental reports, luminescence charts, and raw data files (if requested).
Q5: Do I need to provide cell lines?
A: No. We offer commonly used plant and mammalian cell lines for your assays.