1. Service Overview
Yeast Two-Hybrid (Y2H) is a widely used protein–protein interaction detection technique. By fusing the DNA-binding domain (BD) and activation domain (AD) with target proteins in yeast cells, direct interactions between proteins can be identified and validated.
YBioHub provides professional Y2H services, from single-protein testing to protein library screening, helping researchers quickly discover new protein interactions and signaling pathway relationships.
2. Principle
- Bait protein (Bait): fused with DNA-binding domain (BD)
- Prey protein (Prey): fused with transcriptional activation domain (AD)
When the bait protein interacts with the prey protein, BD and AD are reconstituted, activating downstream reporter genes (e.g., HIS3, ADE2, or LacZ).
Interaction can be detected by yeast growth on selective media or enzymatic activity, indicating protein–protein interaction.
3. Experimental Workflow (Simplified)
- Gene cloning & vector construction: clone target protein sequences into BD and AD fusion vectors
- Yeast transformation: introduce fusion vectors into suitable yeast strains
- Screening & validation: grow yeast on selective media and detect growth or reporter activity
- Data analysis: analyze interaction results and generate a detailed experimental report
4. Applications
- Identification of protein–protein interactions
- Signaling pathway and molecular mechanism studies
- Functional protein screening and validation
- Support for crop improvement and molecular breeding research
5. Service Advantages (YBioHub)
- 📌 Professional team: experienced molecular biology and yeast experiment specialists
- 📌 Versatile systems: suitable for single-protein testing and protein library screening
- 📌 High throughput & reliability: fast and accurate interaction results
- 📌 Complete data: deliver detailed experimental reports, images, and raw data
6. Deliverables
- Yeast growth or reporter activity images
- Experimental analysis report
- Raw data files (upon request)
7. FAQ
Q1: I only have the protein sequence, not the vector. Can I still order this service?
A: Yes. We provide a one-stop service from gene cloning to vector construction.
Q2: How long does the experiment usually take?
A: Typically about two months, depending on the experiment design and sample quantity.
Q3: Can multiple proteins be screened simultaneously?
A: Yes. We offer protein library screening to discover new protein interactions.
Q4: What will I receive as the final results?
A: You will receive an experimental report, interaction analysis images, and raw data (if required).
Q5: Do I need to provide a library?
A: No. We have multiple cDNA libraries available for free use.