Protein-Protein Interaction Experiments
Yeast Two-Hybrid (Y2H)
The yeast two-hybrid system allows researchers to screen for protein-protein interactions in a high-throughput manner. It is widely used to identify interaction partners and build protein interaction networks in plants.
Bimolecular Fluorescence Complementation (BiFC)
BiFC enables direct visualization of protein-protein interactions in living plant cells. By fusing proteins to complementary fragments of a fluorescent protein, interactions reconstitute fluorescence, confirming functional interaction in vivo.
Co-Immunoprecipitation (Co-IP)
Co-IP is used to detect protein complexes in plant tissues or cell extracts. It provides evidence for physical interactions between proteins under native conditions and can be combined with western blotting for validation.
Immunoprecipitation-Mass Spectrometry (IP-MS)
IP-MS combines immunoprecipitation with mass spectrometry to identify protein partners in complex samples. It is powerful for discovering novel interaction networks and protein complexes in plants.
Protein-Nucleic Acid Interaction Experiments
Electrophoretic Mobility Shift Assay (EMSA)
EMSA is used to study protein-DNA or protein-RNA interactions. By observing shifts in mobility of nucleic acids in a gel when bound to proteins, researchers can confirm binding and estimate affinity.
Yeast One-Hybrid (Y1H)
Y1H allows the identification of transcription factors that bind specific DNA sequences. It is used to screen for protein-DNA interactions in a yeast system, providing insights into transcriptional regulation in plants.
Dual-Luciferase Assay
This assay measures promoter activity and transcriptional regulation by using two luciferases. It provides a quantitative and sensitive method to study gene expression modulation by transcription factors.
Luciferase Complementation Assay (LCA)
LCA detects protein-protein or protein-DNA interactions using split luciferase fragments. Interaction of proteins or binding to DNA restores luciferase activity, allowing in vivo detection of molecular interactions.
For inquiries about experimental services, contact us via email: ybiohub@outlook.com.