1. Service Overview
YBioHub provides professional DNA sequencing services for molecular biology and genetic research.
We specialize in Sanger sequencing for plasmid verification, gene identification, mutation detection, and sequence confirmation with high accuracy and fast turnaround.
2. Core Principles
DNA sequencing determines the precise order of nucleotides in a DNA molecule, enabling researchers to validate genetic constructs and study gene structure.
- Sanger sequencing for high-accuracy gene validation
- Plasmid and PCR product verification
- Mutation and SNP detection
- Sequence alignment and analysis
3. Experimental Workflow
- Sample preparation (plasmid, PCR product, or genomic DNA)
- Primer design or validation
- Sanger sequencing reaction
- Capillary electrophoresis analysis
- Sequence alignment and quality control
- Final report generation
4. Applications
- Plasmid construct verification
- Gene cloning confirmation
- Mutation and SNP identification
- PCR product validation
- Molecular biology research support
5. Service Advantages (YBioHub)
- 📌 High-accuracy Sanger sequencing
- 📌 Fast turnaround time
- 📌 Free basic sequence analysis
- 📌 Supports multiple sample types
6. Deliverables
- Raw sequencing chromatogram files
- Cleaned sequence data
- Alignment and annotation report
- Mutation analysis (if applicable)
7. FAQ
Q1: What samples can be sequenced?
Plasmids, PCR products, and genomic DNA are all supported.
Q2: How long does sequencing take?
Typically 2–5 working days depending on sample type.
Q3: Do you provide primer design?
Yes, we can design or validate primers for sequencing.
Q4: What type of sequencing do you use?
We primarily use Sanger sequencing for high accuracy and reliability.
Q5: Can you analyze mutations?
Yes, we provide mutation detection and sequence comparison analysis.
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