1. Service Overview
YBioHub provides professional recombinant protein expression services for molecular biology and biotechnology research.
We offer multiple expression systems including E. coli, yeast, and plant-based systems to achieve efficient and scalable protein production.
2. Core Principles
Protein expression involves introducing a target gene into an expression system to produce functional recombinant proteins.
- Gene-to-protein expression system design
- Codon optimization for high expression efficiency
- Induction of protein expression in host cells
- Protein purification and quality validation
3. Experimental Workflow
- Gene cloning into expression vector
- Vector transformation into host system
- Expression induction (IPTG, etc.)
- Protein extraction and solubility testing
- Purification (His-tag, GST-tag, etc.)
- Protein quality analysis (SDS-PAGE, Western blot)
4. Applications
- Functional protein studies
- Enzyme activity analysis
- Antibody production
- Structural biology research
- Biotechnology and synthetic biology
5. Service Advantages (YBioHub)
- 📌 Multiple expression systems supported
- 📌 High-yield recombinant protein production
- 📌 Purified protein available upon request
- 📌 Fast and reliable experimental workflow
6. Deliverables
- Expression vector and construct confirmation
- Protein expression data
- Purified protein samples (optional)
- Quality analysis report (SDS-PAGE / WB)
7. FAQ
Q1: Which expression systems are supported?
E. coli, yeast, plant and other customized systems are available.
Q2: Can you optimize protein expression?
Yes, we provide codon optimization and expression condition optimization.
Q3: Do you provide purified proteins?
Yes, protein purification services are available upon request.
Q4: How long does the service take?
Typically 2–6 weeks depending on protein complexity.
Q5: Can you test protein solubility?
Yes, we evaluate soluble and insoluble expression fractions.
🔬 Explore More Molecular Biology Services:
Molecular Biology Services