1. Service Overview
YBioHub provides professional PCR and qPCR analysis services for gene amplification and gene expression studies.
Our platform supports DNA and RNA-based quantitative analysis to help researchers accurately evaluate gene expression levels across different biological systems.
2. Core Principles
PCR (Polymerase Chain Reaction) is a technique used to amplify specific DNA sequences, while qPCR (quantitative PCR) enables real-time quantification of nucleic acids.
- Specific amplification of target DNA or cDNA
- Fluorescence-based real-time detection (SYBR Green / TaqMan)
- Quantification of gene expression levels
- High sensitivity and reproducibility
3. Experimental Workflow
- Sample preparation (DNA or RNA extraction)
- Primer design for target genes
- PCR or reverse transcription (RT-PCR) setup
- Amplification and real-time detection
- Data normalization and analysis
- Expression level interpretation
4. Applications
- Gene expression analysis
- Validation of RNA-seq results
- Pathway and functional gene studies
- Mutation detection
- Transgene expression verification
5. Service Advantages (YBioHub)
- 📌 High sensitivity and accurate quantification
- 📌 Optimized primer design support
- 📌 Fast and reproducible experimental workflow
- 📌 Applicable to plant, animal and microbial systems
6. Deliverables
- Raw qPCR amplification data
- Gene expression analysis report
- Standard curve (if required)
- Data interpretation summary
7. FAQ
Q1: What samples can be used for qPCR?
DNA, RNA (after reverse transcription), plant tissues, animal cells and microbial samples are all supported.
Q2: Do you provide primer design?
Yes, we provide optimized primer design based on your gene sequences.
Q3: What is the difference between PCR and qPCR?
PCR is used for amplification, while qPCR allows real-time quantification of DNA/RNA levels.
Q4: How long does the analysis take?
Typically 3–7 days depending on project complexity.
Q5: Can you analyze gene expression across conditions?
Yes, we can compare expression levels across different treatments or conditions.