1. Service Overview
The HTRF (Homogeneous Time-Resolved Fluorescence) assay is an advanced technology for detecting protein-protein interactions based on FRET.
Unlike molecular docking or virtual screening, HTRF provides real experimental data rather than computational predictions.
YBioHub introduces HTRF into plant protein interaction research, enabling rapid, reliable, and cost-effective screening.
2. Why Choose HTRF Over Molecular Docking?
- ✔ Experimental validation instead of prediction
- ✔ Detects real molecular interactions
- ✔ Higher reliability than in silico methods
- ✔ Quantitative interaction data
- ✔ Suitable for weak and transient interactions
Spend docking-level budget, get experimental results.
3. Key Advantages
- 🚀 Ultra-fast turnaround: results in days instead of weeks
- 💰 Extremely cost-effective: comparable to virtual screening
- 🧪 No protein purification required: simplified workflow
- 📊 Quantitative output: precise interaction measurement
- 🔬 High sensitivity: detects weak interactions missed by Y2H
4. Experimental Workflow (Simplified)
- Gene design or sequence submission
- Protein expression (cell-based system)
- HTRF detection using tagged antibodies
- Signal measurement and quantitative analysis
5. Applications
- Plant protein-protein interaction screening
- Validation of candidate interacting proteins
- Small molecule–protein interaction studies
- Functional analysis of signaling pathways
6. A New Direction in Plant Research
HTRF has been widely used in drug discovery but is rarely applied in plant biology.
By introducing this technology into plant protein interaction studies, we provide a new experimental alternative to traditional screening methods.
This approach bridges the gap between computational prediction and experimental validation.
7. Deliverables
- HTRF signal data and charts
- Interaction strength analysis
- Complete experimental report
- Raw data (optional)
8. FAQ
Q1: I only have gene sequences. Can I still use this service?
A: Yes. We provide a full workflow from gene to interaction data.
Q2: Do I need purified proteins?
A: No. Our system does not require protein purification.
Q3: How long does it take?
A: Typically within a few days to one week.
Q4: Is this better than molecular docking?
A: Yes. HTRF provides real experimental evidence rather than computational predictions.
Q5: Can this replace Co-IP or Y2H?
A: HTRF is ideal for screening and quantitative analysis, and can be combined with other methods for publication-level validation.