1. Service Overview
YBioHub provides professional gene expression analysis services for molecular biology and functional genomics research.
We specialize in quantitative analysis of gene expression using qPCR and RT-qPCR to study gene regulation, transcriptional activity, and biological responses.
2. Core Principles
Gene expression analysis measures RNA levels to determine how genes are regulated under different biological conditions.
- RNA extraction and quality assessment
- Reverse transcription to cDNA (RT-qPCR)
- Quantitative PCR amplification
- Relative and absolute expression analysis
3. Experimental Workflow
- Sample collection (plant, animal, or cell samples)
- RNA extraction and purity check
- cDNA synthesis (reverse transcription)
- qPCR amplification with specific primers
- Data normalization (housekeeping genes)
- Expression level analysis and reporting
4. Applications
- Gene regulation studies
- Stress response analysis
- Differential expression profiling
- Validation of RNA-seq results
- Functional genomics research
5. Service Advantages (YBioHub)
- 📌 High sensitivity qPCR detection
- 📌 Accurate gene expression quantification
- 📌 Fast experimental turnaround
- 📌 Customized primer design support
6. Deliverables
- Raw qPCR amplification data
- Gene expression analysis report
- Normalized expression charts
- Statistical comparison results
7. FAQ
Q1: What samples are supported?
Plant, animal tissues, and cultured cells are all supported.
Q2: Do you provide primer design?
Yes, we provide primer design and validation services.
Q3: What is the difference between qPCR and RT-qPCR?
RT-qPCR includes reverse transcription from RNA to cDNA before quantification.
Q4: Can you analyze multiple genes at once?
Yes, multiplex and multi-gene analysis are available.
Q5: How long does the analysis take?
Typically 3–7 working days depending on sample size.
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