1. Service Overview
YBioHub provides professional gene cloning and plasmid construction services for molecular biology research and protein expression studies.
We offer a complete workflow from gene design to recombinant plasmid construction, supporting a wide range of expression systems including bacterial, yeast, plant, and mammalian cells.
2. Core Principles
Gene cloning is the process of inserting a target gene into a vector to generate recombinant DNA for downstream applications.
- Gene sequence optimization for expression efficiency
- Vector selection based on experimental purpose
- Restriction enzyme or seamless cloning strategies
- Verification via sequencing and functional testing
3. Experimental Workflow
- Gene sequence design and optimization
- Vector selection and construction strategy design
- Gene synthesis or PCR amplification
- Ligation or seamless assembly
- Transformation and screening
- Plasmid extraction and sequencing validation
4. Applications
- Protein expression and purification
- Functional gene studies
- Promoter and regulatory element analysis
- Gene overexpression or knock-in studies
- Biotechnology and synthetic biology research
5. Service Advantages (YBioHub)
- 📌 One-stop gene to plasmid construction service
- 📌 Multiple expression systems supported
- 📌 High cloning efficiency and accuracy
- 📌 Fast turnaround and sequencing validation included
6. Deliverables
- Verified recombinant plasmid DNA
- Sequencing confirmation report
- Cloning workflow documentation
- Optional expression validation data
7. FAQ
Q1: Can I provide only a gene sequence?
Yes, we can perform full gene synthesis and cloning starting from sequence information.
Q2: Which vectors are supported?
We support a wide range of bacterial, yeast, plant, and mammalian expression vectors.
Q3: How long does the service take?
Typically 2–4 weeks depending on gene complexity and vector design.
Q4: Is sequencing included?
Yes, all constructs are verified by Sanger sequencing before delivery.
Q5: Can you customize expression systems?
Yes, we can design custom expression systems based on your research needs.