1. Service Overview
YBioHub provides professional ATAC-seq services for genome-wide profiling of chromatin accessibility in plant and model biological systems.
This service helps researchers identify active regulatory regions such as promoters, enhancers, and open chromatin regions associated with gene regulation.
2. Core Principles
ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a high-throughput method to map open chromatin regions.
- Tn5 transposase inserts sequencing adapters into accessible chromatin
- Open chromatin regions are preferentially tagged and amplified
- Sequencing reveals genome-wide accessibility landscape
3. Experimental Workflow
- Sample preparation (plant tissue or cells)
- Nuclei isolation
- Tn5 transposase tagmentation
- Library amplification
- High-throughput sequencing
- Bioinformatics analysis (peak calling & annotation)
4. Applications
- Chromatin accessibility profiling
- Enhancer and promoter identification
- Gene regulation studies
- Developmental biology research
- Plant stress response regulation
5. Service Advantages (YBioHub)
- 📌 High-quality chromatin accessibility profiling
- 📌 Compatible with plant and model systems
- 📌 Integrated sequencing and bioinformatics analysis
- 📌 Optimized low-input sample handling
6. Deliverables
- Raw sequencing data (FASTQ)
- Peak calling results
- Genome annotation of accessible regions
- Data visualization and report
7. FAQ
Q1: What sample types are supported?
Plant tissues, cultured cells, and other eukaryotic samples.
Q2: What is the advantage of ATAC-seq?
It requires low input and provides high-resolution chromatin accessibility maps.
Q3: Do you provide bioinformatics analysis?
Yes, including peak calling, annotation, and pathway enrichment analysis.
Q4: How long does the service take?
Typically 3–6 weeks depending on sample quality and sequencing depth.
Q5: Can it be used for plant research?
Yes, we support plant chromatin accessibility studies and regulatory analysis.